Production of polypeptides

专利名称：Production of polypeptides

发明人：Denis Drapeau,Yen-Tung Luan,James R.

Mercer,Wenge Wang,Daniel R. Lasko

申请号：US11213308申请日：20050825公开号：US07294484B2公开日：20071113

摘要：An improved system for large scale production of proteins and/or polypeptidesin cell culture, particularly in media characterized by one or more of: i) a cumulative aminoacid concentration greater than about 70 mM; ii) a molar cumulative glutamine tocumulative asparagine ratio of less than about 2; iii) a molar cumulative glutamine tocumulative total amino acid ratio of less than about 0.2; iv) a molar cumulative inorganicion to cumulative total amino acid ratio between about 0.4 to 1; or v) a combined

cumulative glutamine and cumulative asparagine concentration between about 16 and 36mM, is provided. The use of such a system allows high levels of protein production andlessens accumulation of certain undesirable factors such as ammonium and/or lactate.Additionally, culture methods including a temperature shift, typically including a decreasein temperature when the culture has reached about 20-80% of it maximal cell density, areprovided. Alternatively or additionally, the present invention provides methods such that,after reaching a peak, lactate and/or ammonium levels in the culture decrease over time.

申请人：Denis Drapeau,Yen-Tung Luan,James R. Mercer,Wenge Wang,Daniel R. Lasko

地址：Salem NH US,Chelmsford MA US,Derry NH US,North Chelmsford MAUS,Medford MA US

国籍：US,US,US,US,US

代理机构：Choate, Hall & Stewart, LLP

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