DNA MOLECULES AND METHODS

专利名称：DNA MOLECULES AND METHODS发明人：MINTON, NIGEL, PETER,HEAP, JOHN,

TIMOTHY

申请号：GB2007002308申请日：20070621

公开号：WO2007148091A3公开日：20080703

摘要：The present application discloses a DNA molecule comprising a modified GroupII intron which does not express the intron-encoded reverse transcriptase but whichcontains a modified selectable marker gene in the reverse orientation, wherein themarker gene comprises a Group I intron in forward orientation of causing expression in abacteria cell of the class Clostridia and wherein the DNA molecule comprises sequencesthat allow for the insertion of the RNA transcript of the Group II intron in the

chromosome of a bacterial cell of the class Clostridia. A method of introducing a nucleicacid molecule into a site of a DNA molecule in a bacterial cell of the class Clostridia isalso provided. The DNA molecule and the method are useful for making mutationsClostridium app.

申请人：MORVUS TECHNOLOGY LTD,MINTON, NIGEL, PETER,HEAP, JOHN, TIMOTHY

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