USE OF FLUORESCENT MOLECULAR BEACONS IN THE DETECT

专利名称：USE OF FLUORESCENT MOLECULAR

BEACONS IN THE DETECTION OFMETHYLATED NUCLEIC ACIDS

发明人：KAY, Peter, H.申请号：EP00904696.2申请日：20000201公开号：EP1151138A1公开日：20011107

摘要：The present invention provides a method for detecting methylated nucleic acidscomprising the steps of: 1) contacting a nucleic acid sample suspected of containingmethylated nucleotides with an oligonucleotide sequence under suitable conditions fornucleic acid hybridization, said oligonucleotide sequence characterised in that, (i) itcomprises a first stem labeled with a fluorophore moiety, a loop sequence having aregion of nucleotides complementary to at least a region of the nucleic acid sample,which region is susceptible to methylation, and a second stem labeled with a quenchermoiety that is capable of quenching the fluorophore moiety when in spatial proximity tothe fluorophore moiety; and (ii) the nucleotides forming the first stem are capable ofmoving into spatial proximity with the nucleotides forming the second stem when theprobe is dissociated from the nucleic acid sample; 2) altering the hybridization conditionssuch that the oligonucleotide probe dissociates from methylated DNA but remainshybridized to methylated DNA; and 3) measuring the change in fluorescence.

申请人：THE UNIVERSITY OF WESTERN AUSTRALIA

地址：Stirling Highway Nedlands,W.A. 6009 AU

国籍：AU

代理机构：Khoo, Chong-Yee

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